Active human PHGDH full length protein
| Name | Active human PHGDH full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198455 |
| Category | Protein |
| Prices | $785.00 |
| Sizes | 100 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | His tag C-Terminus |
| Purity | > 80 % by SDS-PAGE. |
| Bioactivity | Specific Activity: ≥80 pmol/min/µg Assay Conditions: The reaction mixture contained 100 mM Tris-HCl, pH 8.8, 400 mM NaCl 2 , 0.2 mM DTT, 2.5 mM 3-phospho-D-glyceric acid, 1 mM NAD, and various amount of ab198455 in a final volume of 50 µL. Reactions were initiated with the addition of ab198455, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature. |
| SwissProt/Accession | O43175 |
| Gene | PHGDH |
| Residue | 1 to 533 |
| Sequence | MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEG LIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPN GNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGI LGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLC DFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQ SGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIA VQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSP KGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEA GLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNG AVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLV SDGETWHVMGISSLLPSLEAWKQHVTEAFQFHFHHHHHH |
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