All lanes : Anti-NPFF1 Receptor antibody ( ab45350 ) at 2 µg/ml Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with Human NPFF1 Receptor peptide (ab45349) at 1 µg/ml Lysates/proteins at 15 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing conditions. Predicted band size : 48 kDa Observed band size : 64 kDa ( why is the actual band size different from the predicted? )