| Name |
Active human PPIH full length protein
|
| Supplier |
Abcam
|
| Catalog |
ab78874
|
| Category |
Protein
|
| Prices |
$192.00
|
| Sizes |
50 µg
|
| Applications |
SDS-PAGE FA
|
| Species Reactivities |
Human
|
| Nature |
Recombinant
|
| Source |
E. coli
|
| Purity |
> 95 % by SDS-PAGE. ab78874 is purified using conventional chromatography techniques.
|
| Bioactivity |
Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. Add 10 µl of recombinant Cyclophikin H (PPIH) protein with 1 µg in assay buffer. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) Record the increase in A405 nm for 30 minutes at 25°C.
|
| Endotoxin |
< 1.000 Eu/µg
|
| SwissProt/Accession |
O43447
|
| Gene |
PPIH
|
| Sequence |
MAVANSSPVNPVVFFDVSIGGQEVGRMKIELFADVVPKTAENFRQFCTGE FRKDGVPIGYKGSTFHRVIKDFMIQGGDFVNGDGTGVASIYRGPFADENF KLRHSAPGLLSMANSGPSTNGCQFFITCSKCDWLDGKHVVFGKIIDGLLV MRKIENVPTGPNNKPKLPVVISQCGEM
|
| Supplier Page |
Shop
|
15% SDS-PAGE showing ab78874 at approximately 19kDa (3µg).