| Name |
Active human PPIL3 full length protein
|
| Supplier |
Abcam
|
| Catalog |
ab113119
|
| Category |
Protein
|
| Prices |
$302.00
|
| Sizes |
100 µg
|
| Applications |
MS FA SDS-PAGE
|
| Species Reactivities |
Human
|
| Nature |
Recombinant
|
| Source |
E. coli
|
| Tag/Conjugation |
His tag N-Terminus
|
| Purity |
> 90 % by SDS-PAGE. ab113119 was purified using conventional chromatography.
|
| Bioactivity |
Specific activity is > 280 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. Add 10 µl of recombinant PPIL3 protein with 1 µg in assay buffer. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) Record the increase in A405 nm for 30 minutes at 25°C.
|
| SwissProt/Accession |
Q9H2H8
|
| Gene |
PPIL3
|
| Residue |
1 to 161
|
| Sequence |
MGSSHHHHHHSSGLVPRGSHMSVTLHTDVGDIKIEVFCERTPKTCENFLA LCASNYYNGCIFHRNIKGFMVQTGDPTGTGRGGNSIWGKKFEDEYSEYLK HNVRGVVSMANNGPNTNGSQFFITYGKQPHLDMKYTVFGKVIDGLETLDE LEKLPVNEKTYRPLNDVHIKDITIHANPFAQ
|
| Supplier Page |
Shop
|
15% SDS-PAGE analysis of ab113119 (3µg)