Active human PRMT5 + MEP50 full length protein
| Name | Active human PRMT5 + MEP50 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198151 |
| Category | Protein |
| Prices | $1,281.00 |
| Sizes | 20 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Mammalian |
| Tag/Conjugation | DDDDK tag N-Terminus |
| Purity | >= 75 % by SDS-PAGE. |
| Bioactivity | Assay Conditions: 50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 10-100 ng methyltransferase PRMT5) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence. |
| SwissProt/Accession | O14744 |
| Gene | PRMT5 |
| Residue | 2 to 637 |
| Sequence | AAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFK REFIQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLSPWIRPDSKVEKIRR NSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMF WMRVPLVAPEDLRDDIIENAPTTHTEEYSGEEKTWMWWHNFRTLCDYSKR IAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQ RLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFA KGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPE EEKDTNVQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTL ENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLD GAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMP YVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGF AGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVR FWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL |
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