Bioactivity |
ab190039 is covalently coupled to agarose beads via primary amines allowing for a fully functional C terminus. It is useful for isolation and capture of Sumo 2 interacting proteins such as the SUMO activating E1 enzyme, the SUMO carrier enzyme Ubch9, SUMO E3 ligases, SENPs and other proteins/enzymes that have an affinity for SUMO proteins. Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.
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