TrkB protein (Fc Chimera Active)
| Name | TrkB protein (Fc Chimera Active) |
|---|---|
| Supplier | Abcam |
| Catalog | ab83565 |
| Category | Protein |
| Prices | $302.00 |
| Sizes | 20 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | ab83565 chimera bound to protein A sepharose beads is able to pull down its ligand, BDNF. |
| SwissProt/Accession | Q16620 |
| Gene | NTRK2 |
| Residue | 32 to 428 |
| Sequence | Theoretical Sequence: CPTSCKCSASRIWCSDPSPGIVAFPRLEPNSVDPENITE IFIANQK RLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQ HINFT RNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAK SSP DTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSI T LSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSG KQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTV K GNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTH MNN GDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYED YGTAA NDIGDTTNRSNEIPSTDVTDKTGRIPKVDKKVEPKSCDKTH TCPPCPA PELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHED PEVKFNWYV DGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNG KEYKCKVSNKA LPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQ VSLTCLVKGFYPS DIAVEWESNGQPENNYKTTPPVLDSDGSFFLYS KLTVDKSRWQQGNVF SCSVMHEALHNHYTQKSLSLSPGK |
| Supplier Page | Shop |
Product images
Lane 1 – MW markers; Lane 2 – ab83565; Lane 3 – ab83565 treated with PNGase F to remove potential N-linked glycans. 10 µg protein loaded per lane. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Band in lane 3 at 35 kDa is PNGase F.
A sample of ab83565 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms of TrkB FcB Chimera. Spots within the spot train were cut from the gel and identified as TrkB FcB Chimera by protein mass fingerprinting.
Densitometry scan, derived from the 2D gel image, demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates theoretical pI and MW of the protein.