Active human UCH37 full length protein
| Name | Active human UCH37 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab108375 |
| Category | Protein |
| Prices | $239.00, $883.00 |
| Sizes | 100 µg, 500 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | His tag N-Terminus |
| Purity | > 90 % by SDS-PAGE. ab108375 is purified using conventional chromatography techniques. |
| Bioactivity | Specific activity: > 500 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C. Activity Assay: Prepare a 100ul of recombinant UCH-L5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) |
| SwissProt/Accession | Q9Y5K5 |
| Gene | UCHL5 |
| Residue | 1 to 329 |
| Sequence | MGSSHHHHHHSSGLVPRGSH MTGNAGEWCLMESDPGVFTELIKGFGCRGA QVEEIWSLEPENFEKLKPVHGLIFLFKWQPGEEPAGSVVQDSRLDTIFFA KQVINNACATQAIVSVLLNCTHQDVHLGETLSEFKEFSQSFDAAMKGLAL SNSDVIRQVHNSFARQQMFEFDTKTSAKEEDAFHFVSYVPVNGRLYELDG LREGPIDLGACNQDDWISAVRPVIEKRIQKYSEGEIRFNLMAIVSDRKMI YEQKIAELQRQLAEEEPMDTDQGNSMLSAIQSEVAKNQMLIEEEVQKLKR YKIENIRRKHNYLPFIMELLKTLAEHQQLIPLVEKAKEKQNAKKAQETK |
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Product images
15% SDS-PAGE analysis of ab108375 (3µg)