Name | Active human WHSC1/NSD2 protein fragment |
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Supplier | Abcam |
Catalog | ab198120 |
Category | Protein |
Prices | $858.00 |
Sizes | 50 µg |
Applications | FA SDS-PAGE |
Species Reactivities | Human |
Nature | Recombinant |
Source | E. coli |
Tag/Conjugation | GST tag N-Terminus |
Purity | >= 82 % by SDS-PAGE. |
Bioactivity | Specific Activity: 0.036 pmol/min/μg Assay Conditions: Add 50 μL reaction mix (100 μM S-adenosylmethionine, and 50-250 ng WHSC1/NSD2 in HMT buffer 7) to wells coated with the substrate. Incubate at 37 o C for 2 hours. Add antibody against methylated K36 residue of histone H3, incubate 1 hour. Then, add secondary HRP-labeled antibody and incubate 30 minutes. Finally, add HRP chemiluminescent substrates and read luminescence. |
SwissProt/Accession | O96028 |
Gene | WHSC1 |
Residue | 941 to 1240 |
Sequence | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEGDRGSRYQGVRGIGRVFK NALQEAEARFREIKLQREARETQESERKPPPYKHIKVNKPYGKVQIYTAD ISEIPKCNCKPTDENPCGFDSECLNRMLMFECHPQVCPAGEFCQNQCFTK RQYPETKIIKTDGKGWGLVAKRDIRKGEFVNEYVGELIDEEECMARIKHA HENDITHFYMLTIDKDRIIDAGPKGNYSRFMNHSCQPNCETLKWTVNGDT RVGLFAVCDIPAGTELTFNYNLDCLGNEKTVCRCGASNCSGFLGDRPKTS TTLSSEEKGKKTKKKTRRRRAKGEGKRQSED |
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