Rat IGF-1

• Supplier: ABBIOTEC
• Catalog: 600223
• Category: Protein
• Prices: $250.00
• Sizes: 50 µg
• Species Reactivities: Rat
• Nature: Recombinant
• Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Purity: Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Bioactivity: Rat IGF-1 is fully biologically active when compared to standards. The ED50, was calculated 2 methods: 1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be less then 30 ng/ml. 2. Type 1 IGF receptor binding assay ED50 was found to be less then 10 ng/ml.
• Gene: Igf1
• Sequence: GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA
• Description: Insulin-like growth factor I (IGF1) is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. IGF1 has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. Recombinant Rat IGF1 produced in E. coli is a single non-glycosylated polypeptide chain containing 70 amino acids with a MW of 7,687 Da.