Recombinant BRWD1 (1310-1430) protein
| Name | Recombinant BRWD1 (1310-1430) protein |
|---|---|
| Supplier | ActiveMotif |
| Catalog | 31440 |
| Category | Protein |
| Prices | $395.00, $2,600.00 |
| Sizes | 100 μg, 1 mg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Gene | BRWD1 |
| Description | The protein corresponding to amino acids 1310-1430 that contains the bromodomain sequence of human BRWD1 protein (accession number NP_061836.2) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with a molecular weight of 18.97 kDa |
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Product images
Recombinant BRWD1 (1310-1430) protein gel. BRWD1 (1310-1430) protein was run on an SDS-PAGE gel and stained with Coomassie blue.
HTRF assay for BRWD1 (1310-1430) activity assay. 3. 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRWD1 (1310-1430) protein in reaction buffer including 50mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.
HTRF Assay for Recombinant BRWD1 (1310-1430) protein activity. 3 µM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRWD1 (1310-1430) protein in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.