Recombinant PBRM1 (464-605) protein

• Supplier: ActiveMotif
• Catalog: 31384
• Category: Protein
• Prices: $395.00, $3,500.00
• Sizes: 100 µg, 1 mg
• Species Reactivities: Human
• Nature: Recombinant
• Source: E. coli
• Gene: PBRM1
• Description: The peptide corresponding to amino acids 464 - 605 that contains the bromodomain sequences of PBRM1 (accession number NM_018165.4) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 22.5 kDa

Product images

Recombinant PBRM1 (464-605) protein gel. PBRM1 (464-605) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.

Recombinant PBRM1 (464-605) activity using AlphaScreen. PBRM1 (464-605) was used in an AlphaScreen® assay to generate a titration curve demonstrating the signal response in the presence of modified peptide substrate at increasing protein concentrations. This data was generated and kindly provided courtesy of ChemPartner.

HTRF Assay for Recombinant PBRM1 (464-605) activity. 3 µM histone peptide H4K5/8/12/16 (4Ac) was incubated with PBRM1 (464-605) protein in reaction buffer including 50mM HEPES-NaOH pH 7. 0, 0. 1% BSA overnight at room temperature. Anti-Flag antibody was used to detect reaction products.