Recombinant SP140L (401-580) protein

• Supplier: ActiveMotif
• Catalog: 31437
• Category: Protein
• Prices: $395.00
• Sizes: 100 μg
• Species Reactivities: Human
• Nature: Recombinant
• Source: E. coli
• Gene: SP140L
• Description: The protein corresponding to amino acids 401-580 that contains the bromodomain sequence of human SP140L protein (accession number NP_612411.4) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with a molecular weight of 26.3 kDa

Product images

Recombinant SP140L (401-580) protein gel. SP140L (401-580) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.

Recombinant SP140L (401-580) activity using AlphaScreen. SP140L (401-580) was used in an AlphaScreen® assay to generate a titration curve demonstrating the signal response in the presence of modified peptide substrate at increasing protein concentrations. This data was generated and kindly provided courtesy of ChemPartner.

HTRF Assay for Recombinant SP140L (401-580) protein activity . 3 µM histone peptide H3K14ac was incubated with SP140L (401-580) in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.