Recombinant TAF1L (1402-1521) protein gel. TAF1L (1402-1521) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.
HTRF Assay for Recombinant TAF1L (1402-1521) protein activity. 3 µM histone peptide H4K5/8/12/16(4ac) was incubated with TAF1L (1402-1521) in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.
HTRF Assay for Recombinant TAF1L (1402-1521) protein activity. 3 µM histone peptide H4K5/8/12/16(4ac) was incubated with TAF1L (1402-1521) in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.