Recombinant JMJD1B / KDM3B protein
| Name | Recombinant JMJD1B / KDM3B protein |
|---|---|
| Supplier | ActiveMotif |
| Catalog | 31429 |
| Category | Protein |
| Prices | $395.00 |
| Sizes | 20 μg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus |
| Gene | KDM3B |
| Description | Recombinant JMJD1B / KDM3B (accession number NP_057688.2) was expressed in Sf9 cells and contains an N-terminal 6×His tag and FLAG tag with a molecular weight of 195.4 kDa |
| Supplier Page | Shop |
Product images
Recombinant JMJD1B / KDM3B protein gel. JMJD1B / KDM3B protein was run on an SDS-PAGE gel and stained with Coomassie blue.
JMJD1B / KDM3B activity assay. Recombinant JMJD1B / KDM3B activity measured using a demethylation assay. MALDI-TOF was used for detection.
Recombinant JMJD1B / KDM3B protein activity assay. 3. 3 μM H3K9me2 peptide was incubated with recombinant JMJD1B / KDM3B protein in reaction buffer for 1 hours at room temperature. JMJD1B / KDM3B enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me0) was measured using H3K9me0-specific antibody.