Recombinant JMJD2D / KDM4D protein gel. JMJD2D / KDM4D protein was run on an SDS-PAGE gel and stained with Coomassie blue.
JMJD2D / KDM4D activity assay. Recombinant JMJD2D / KDM4D activity measured using a demethylation assay. MALDI-TOF was used for detection.
Recombinant JMJD2D / KDM4D activity using AlphaLISA. JMJD2D / KDM4D was used in an AlphaLISA assay to determine enzyme titration. An IC50 dose response assessment of reference compounds 2,4-PDCA and N-OG is shown. This data was generated and kindly provided courtesy of ChemPartner.