Recombinant KDM1B / LSD2 protein gel. Recombinant KDM1B / LSD2 run on an SDS-PAGE gel and stained with Coomassie blue.
KDM1B / LSD2 activity assay. Recombinant KDM1B / LSD2 activity measured using a demethylation assay. MALDI-TOF was used for detection.
Recombinant KDM1B / LSD2 protein activity assay. 3. 3 μM H3K4me2 (1-27aa) peptide was incubated with KDM1B / LSD2 in reaction buffer for 1 hour at room temperature. KDM1B / LSD2 enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K4me0) was measured using H3K4me0-specific antibody.