All lanes : Monoamine Oxidase A peptide (141-154) (ab45814) at 0.3 µg/ml Lane 1 : Human Heart Lysate Lane 2 : Human Heart Lysate with Immunising peptide Lysates/proteins at 35 µg per lane. developed using the ECL technique Observed band size : 70 kDa ( why is the actual band size different from the predicted? ) Additional bands at : 85 kDa (possible non-specific binding).