Lane 1 : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody ( ab12108 ) at 0.3 µg/ml Lane 2 : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody ( ab12108 ) at 1 µg/ml Lanes 3 - 5 : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody ( ab12108 ) at 3 µg/ml Lane 1 : mouse brain extract Lane 2 : mouse brain extract Lane 3 : mouse brain extract Lane 4 : mouse brain extract with Mouse Glutamate Receptor 1 (AMPA subtype) (phospho T840) peptide ( ab12399 ) at 2 µg/ml Lane 5 : mouse brain extract with Mouse Glutamate Receptor 1 (AMPA subtype) (unmodified ) peptide (ab12434) at 2 µg/ml Performed under reducing conditions. Predicted band size : 102 kDa Observed band size : 105 kDa ( why is the actual band size different from the predicted? ) Additional bands at : 120 kDa (possible cross reactivity),57 kDa (possible IgG). Exposure time : 5 minutes Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom