Lane 1 : Anti-PSD95 (phospho S415) antibody ( ab16492 ) at 0.3 µg/ml Lane 2 : Anti-PSD95 (phospho S415) antibody ( ab16492 ) at 1 µg/ml Lanes 3 - 4 : Anti-PSD95 (phospho S415) antibody ( ab16492 ) at 3 µg/ml Lane 1 : mouse hippocampus lysate Lane 2 : mouse hippocampus lysate Lane 3 : mouse hippocampus lysate Lane 4 : mouse hippocampus lysate with Mouse PSD95 (phospho S415) peptide (ab17214) at 3 µg/ml Lysates/proteins at 10 µg per lane. Predicted band size : 105 kDa Observed band size : ~105 kDa ( why is the actual band size different from the predicted? ) Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom