Lane 1 : Anti-PSD95 antibody ( ab12093 ) at 0.3 µg/ml Lane 2 : Anti-PSD95 antibody ( ab12093 ) at 1 µg/ml Lanes 3 - 4 : Anti-PSD95 antibody ( ab12093 ) at 3 µg/ml Lane 1 : 10µg mouse brain extract/lane Lane 2 : 10µg mouse brain extract/lane Lane 3 : 10µg mouse brain extract/lane Lane 4 : 10µg mouse brain extract/lane with Mouse PSD95 peptide (ab12419) at 1 µg/ml Performed under reducing conditions. Predicted band size : 105 kDa Observed band size : ~105 kDa ( why is the actual band size different from the predicted? ) Exposure time : 10 seconds Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom