Lane 1 : Anti-PSD95 (phospho S295) antibody ( ab16495 ) at 0.3 µg/ml Lane 2 : Anti-PSD95 (phospho S295) antibody ( ab16495 ) at 1 µg/ml Lanes 3 - 5 : Anti-PSD95 (phospho S295) antibody ( ab16495 ) at 3 µg/ml Lane 6 : Anti-PSD95 antibody ( ab12093 ) at 0.3 µg/ml Lane 1 : mouse hippocampus lysate Lane 2 : mouse hippocampus lysate Lane 3 : mouse hippocampus lysate Lane 4 : mouse hippocampus lysate with Mouse PSD95 (phospho S295) peptide ( ab17222 ) at 3 µg/ml Lane 5 : mouse hippocampus lysate with Mouse PSD95 peptide (ab17223) at 3 µg/ml Lane 6 : mouse hippocampus lysate Predicted band size : 95 kDa Observed band size : ~105 kDa ( why is the actual band size different from the predicted? ) Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom