All lanes : Anti-CAPS1 antibody ( ab32787 ) at 1 µg/ml Lane 1 : Brain (Rat) Tissue Lysate at 10 µg Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg Lane 3 : Heart (Mouse) Tissue Lysate at 10 µg Lane 4 : Brain (Rat) Tissue Lysate with Rat CAPS1 peptide (ab32786) at 1 µg/ml Lane 5 : Brain (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/ml Lane 6 : Heart (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/ml Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 146 kDa Observed band size : 146 kDa