Name | Active human Acetyl Coenzyme A carboxylase alpha full length protein |
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Supplier | Abcam |
Catalog | ab196428 |
Category | Protein |
Prices | $1,311.00 |
Sizes | 10 µg |
Applications | SDS-PAGE FA |
Species Reactivities | Human |
Nature | Recombinant |
Source | Baculovirus infected sf9 cells |
Tag/Conjugation | His-DDDDK tag C-Terminus |
Purity | >= 70 % by SDS-PAGE. |
Bioactivity | Specific Activity: ≥42.5 pmol/min/µg. A 25 μl Acetyl Coenzyme A carboxylase alpha reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl 2 , 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO 3 and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected. |
SwissProt/Accession | Q13085 |
Gene | ACACA |
Residue | 1 to 2383 |
Sequence | MWWSTLMSILRARSFWKWISTQTVRIIRAVRAHFGGIMDEPSPLAQPLEL NQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLG ISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFG GNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLK ANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHAS ENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGS GLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKAS EGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQIL ADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAK MVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQ IAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSE NPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSW GENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWL DRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPA HTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGG LLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLI QYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGC VLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNV MNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPN VEKSIKKEMAQYASNITSVLCQFPSQQIANI |
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