| Name |
Active rat PDE1B full length protein
|
| Supplier |
Abcam
|
| Catalog |
ab198476
|
| Category |
Protein
|
| Prices |
$1,250.00
|
| Sizes |
10 µg
|
| Applications |
FA SDS-PAGE
|
| Species Reactivities |
Rat
|
| Nature |
Recombinant
|
| Source |
Baculovirus infected sf9 cells
|
| Tag/Conjugation |
His tag N-Terminus , GST tag N-Terminus
|
| Purity |
>= 35 % by SDS-PAGE.
|
| Bioactivity |
Specific Activity: ≥ 6800 pmol/min/µg Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl 2 , 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198476 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
|
| SwissProt/Accession |
Q01064
|
| Gene |
PDE1B
|
| Residue |
1 to 535
|
| Sequence |
MELSPRSPPEMLESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEV NIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWL ASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTAVGPTYS TAVHNCLKNLDVWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPT VFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEI EVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVF RMMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQ QLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAE LGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLTDDD SKSKSQPSFQWRQPSLDVDVGDPNPDVVSFRSTWTKYIQENKQKWKERAA SGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD
|
| Supplier Page |
Shop
|
