| Name | Recombinant Human OTUD3, GST-tagged |
|---|---|
| Supplier | Creative Biomart |
| Catalog | OTUD3-178H |
| Category | Protein |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | GST |
| Purity | >98% by SDS-PAGE |
| Bioactivity | Deubiquitylase Enzyme Assay: The activity of GST-OTUD3 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-OTUD3 was compared confirming the deubiquitylating activity of GST-OTUD3. |
| SwissProt/Accession | Q5T2D3 |
| Gene | OTUD3 |
| Description | Recombinant human OTUD3 (amino acid residues 1-629), fused with N-terminal GST, was expressed in E.coli. |
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