| Name | BRD4 (49-460aa), His, Human |
|---|---|
| Supplier | GenScript |
| Catalog | Z03187-100 |
| Category | Protein |
| Prices | $195.00 |
| Sizes | 100 μg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | His |
| Purity | > 95% by SDS-PAGE and HPLC analysis. |
| Bioactivity | Assay condition: 50 mM HEPES, 50 mM NaCl, and 1 mM CHAPS, pH 7.5. Biotinylated acetyl-histone peptide substrate binding to Bromodomain reaction was performed by adding 200 nM substrate to serially diluted BRD, and incubation for 1 h at RT. Treatment with equivalent detection buffer (2 nM Eu-Anti-His and 40 nM U-light-SA in Assay buffer), and incubation for 1 h at RT subsequently followed. |
| Endotoxin | < 1 EU/μg, determined by LAL method. |
| Gene | BRD4 |
| Sequence | MHHHHHHETS NPNKPKRQTN QLQYLLRVVL KTLWKHQFAW PFQQPVDAVK LNLPDYYKII KTPMDMGTIK KRLENNYYWN AQECIQDFNT MFTNCYIYNK PGDDIVLMAE ALEKLFLQKI NELPTEETEI MIVQAKGRGR GRKETGTAKP GVSTVPNTTQ ASTPPQTQTP QPNPPPVQAT PHPFPAVTPD LIVQTPVMTV VPPQPLQTPP PVPPQPQPPP APAPQPVQSH PPIIAATPQP VKTKKGVKRK ADTTTPTTID PIHEPPSLPP EPKTTKLGQR RESSRPVKPP KKDVPDSQQH PAPEKSSKVS EQLKCCSGIL KEMFAKKHAA YAWPFYKPVD VEALGLHDYC DIIKHPMDMS TIKSKLEARE YRDAQEFGAD VRLMFSNCYK YNPPDHEVVA MARKLQDVFE MRFAKMPDE |
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