Active human AKT1 full length protein
| Name | Active human AKT1 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab79792 |
| Category | Protein |
| Prices | $302.00 |
| Sizes | 10 µg |
| Applications | FA SDS-PAGE WB |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | His tag N-Terminus |
| Bioactivity | The purified AKT1 is activated in vitro using PDK1 enzyme. Specific Activity: 12.32 pmol/min/µg. Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate at room temperature for 1 hour. |
| SwissProt/Accession | P31749 |
| Gene | AKT1 |
| Residue | 1 to 480 |
| Sequence | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. |
| Supplier Page | Shop |
Product images
Kinase assay: Specific activity 12.32 pmol/min/µg
10% SDS-PAGE showing ab79792 at approximately 60.2kDa (7µg).
Anti-AKT1 antibody ( ab91505 ) at 1 µg/ml + Active human AKT1 full length protein (ab79792) at 0.1 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080 ) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Exposure time : 30 seconds