Anti-Creatine Kinase MM antibody [3E5BA5] ( ab110319 ) at 1 µg/ml + Recombinant Creatin Kinase M -type (canine) (ab197789) at 0.1 µg Secondary HRP conjugated Goat anti-mouse IgG (H&L) at 1/5000 dilution developed using the ECL technique Exposure time : 5 minutes Samples run on a 4-20% acrylamide gel, performed under reducing conditions and developed using the ECL technique. Membrane was blocked with 5% Milk in PBS, Primary antibody was incubated in 1% Milk in PBS and secondary antibody was incubated in 1X Blocking buffer ( ab126587 ). Predicted CKM band size: 43.1 kDa Observed CKM band size: 43 kDa