SDS-PAGE: 4-12% Bis-Tris NuPAGE gel Lane 1. 5 µg alpha 1 Antitrypsin (reduced/heated) Lane 2. 10 µg alpha 1 Antitrypsin (reduced/heated) Lane 3. 20 µg alpha 1 Antitrypsin (reduced/heated) Lane 4. Molecular weight markers Lane 5. 5 µg alpha 1 Antitrypsin (non-reduced/no heat) Lane 6. 10 µg alpha 1 Antitrypsin (non-reduced/no heat) Lane 7. 20 µg alpha 1 Antitrypsin (non-reduced/no heat)
Anti-alpha 1 Antitrypsin antibody [B9] ( ab9399 ) at 1 µg/ml + Active human alpha 1 Antitrypsin full length protein (ab91136) at 0.1 µg Secondary Goat Anti-Mouse IgG H&L (HRP) preadsorbed ( ab97040 ) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Exposure time : 30 seconds