All lanes : GOLGA3 peptide (1486-1498) (Carboxyterminal end) (ab45813) at 0.1 µg/ml Lane 1 : HeLa lysate Lane 2 : HeLa lysate with Immunising peptide Lysates/proteins at 35 µg per lane. developed using the ECL technique Observed band size : 170 kDa ( why is the actual band size different from the predicted? ) Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.