Active human B Raf/p50 recombinant protein fragment

Name Active human B Raf/p50 recombinant protein fragment
Supplier Abcam
Catalog ab42596
Category Protein
Prices $321.00
Sizes 10 µg
Applications B/N
Species Reactivities Human
Nature Recombinant
Source Baculovirus infected sf9 cells
Tag/Conjugation His tag N-Terminus
Bioactivity Specific Activity: 12 pmol/min/μg. Activity was tested using 1 μM MEK1 (K97R) as substrate. Assay was performed in a 50 μl reation in 10 mM Tris, pH 7.4, 5 mM MgCl 2 , 0.5 mM EDTA, 0.5 mM DTT, 5 μM ATP, 5 μg MEK1 (K97R) as substrate, and B Raf. 62 ng of B Raf enzyme phosphorylated over 18% of 1 μM MEK1 at room temperature in 1 hr.
SwissProt/Accession P15056
Gene BRAF
Residue 432 to 725
Sequence SEDRNRMKT LGRRDSSDDW EIPDGQITVG QRIGSGSFGT VYKGKWHGDV AVKMLNVTAP TPQQLQAFKN EVGVLRKTRH VNILLFMGYS TKPQLAIVTQ WCEGSSLYHH LHIIETKFEM IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV KIGDFGLATV KSRWSGSHQF EQLSGSILWM APEVIRMQDK NPYSFQSDVY AFGIVLYELM TGQLPYSNIN NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMAECLKKK RDERPLFPQI LASIELLARS LPKIH
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