c-Kit protein (Fc Chimera Active)
| Name | c-Kit protein (Fc Chimera Active) |
|---|---|
| Supplier | Abcam |
| Catalog | ab88349 |
| Category | Protein |
| Prices | $525.00 |
| Sizes | 50 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | Activity: The ED 50 of ab88349 is typically 1-3 ug/ml as measured by its ability to neutralize SCF mediated proliferation using the human growth factor dependent M-07e cell line. |
| SwissProt/Accession | P10721 |
| Gene | KIT |
| Sequence | GSSQPSVSPGEPSPPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEIL DETNENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLV DRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKA G IMIKSVKRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKAVPVVSV SK ASYLLREGEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHH GDF NYERQATLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGF INIF PMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWE DYPKS ENESNIRYVSELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVY VNTKPE ILTYDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSASVLP VDVQTLN SSGPPFGKLVVQSSIDSSAFKHNGTVECKAYNDVGKTSAYF NFAFKGNN KEQIHPHTGSSNTKVDKKVEPKSCDKTHTCPPCPAPELLG GPSVFLFPP KPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEV HNAKTKPREE QYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI EKTISKAKGQP REPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVE WESNGQPENNYK TTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVM HEALHNHYTQKSL SLSPGK |
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Product images
1D SDS-PAGE of ab88349 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab88349 Lane 2: ab88349 treated with PNGase F to remove potential N-linked glycans Lane 3: ab88349 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Appearance of additional band at lower MWt after treatment with PNGase F indicates the presence of N-linked glycans. The subsequent drop in MWt after treatment with a glycosidase cocktail indicates the presence of O-linked glycans.
A sample of ab88349 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg of protein was loaded. Spot trains indicate presence of multiple isoforms of ab88349.
Densitometry of protein isoforms visualised by 2-DE. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.