Active human Caspase-3 full length protein

• Supplier: Abcam
• Catalog: ab52314
• Category: Protein
• Prices: $340.00
• Sizes: 5 µg
• Applications: SDS-PAGE
• Species Reactivities: Human
• Nature: Recombinant
• Source: E. coli
• Purity: > 90 % by SDS-PAGE.
• Bioactivity: Specific Activity:300,000 - 400,000 units/mg. Enzyme Assay: Use at 1 unit/assay. One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37degC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
• SwissProt/Accession: P42574
• Gene: CASP3
• Sequence: Belongs to the peptidase C14A family.

Product images

Lane 1 : Recombinant caspase 3 protein (Active) ab52314 at 2 ng. Lane 2 : Hela-Staurosporine ab52203 at 40 ug. Lane 3 : Jurkat-Staurosporine at 20 ug. Lane 4 : HeLa-Vehicle ab52203 at 40 ug. Lane 5 : Jurkat-Vehicle at 20 ug. Primary : Anti Caspase 3 ( ab32351 ) 1:2000. Secondary : Goat polyclonal to Rabbit IgG ? H&L at 1:10000. Development: ECL with exposure 10 min. Predicted band size : 17 kDa. Migration of observed specific band is indicated with arrow

Lane 1 = Caspase 3 protein (Active) (ab52314) ? 20 ng. Lane 2 = Caspase 9 protein (Active) ( ab52203 ) ? 20 ng. Lane 3 = Extract of HeLa cells treated with vehicle ( ab136806 ) ? 20 ug. Lane 4 = Extract of HeLa cells treated with staurosporine ( ab136806 ) ? 20 ug. SDS PAGE performed under reducing conditions (100mM DTT ? Sample heated at 50?C). Primary : Lanes 1-4: Anti active+pro Caspase 3 antibody ( ab13585 ) at 1 ug/mL. Secondary : Lanes 1-4: Goat anti mouse IgG(H&L)-HRP at 1:10000. Development: ECL with 1 min exposure. Blocking: in 5% Milk + PBS overnight at 4 C. Primary antibody: in 5% Milk + PBS for 2 hours at RT. Secondary antibody: in 5% Milk + PBS for 2 hours at RT. Predicted band size : 17 kDa. Observed band size : 32 kDa and 17 kDa.