SDS-PAGE: 4-12% Bis-Tris NuPAGE gel Lane 1: 5 µg Catalase (reduced/heated) Lane 2: 10 µg Catalase (reduced/heated) Lane 3: 20 µg Catalase (reduced/heated) Lane 4: Molecular weight markers Lane 5: 5 µg Catalase (non-reduced/no heat) Lane 6: 10 µg Catalase (non-reduced/no heat) Lane 7: 20 µg Catalase (non-reduced/no heat)
All lanes : Anti-Catalase antibody - Peroxisome Marker ( ab15834 ) at 5 µg/ml Lane 1 : Active human Catalase full length protein (ab91026) at 0.1 µg Lane 2 : Active human Catalase full length protein (ab91026) at 0.01 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080 ) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Exposure time : 10 seconds