Active human Cathepsin F full length protein

Name Active human Cathepsin F full length protein
Supplier Abcam
Catalog ab198436
Category Protein
Prices $1,065.00
Sizes 50 µg
Applications FA SDS-PAGE
Species Reactivities Human
Nature Recombinant
Source HEK 293 cells
Tag/Conjugation His tag C-Terminus
Purity >90% by SDS-PAGE. Purity varies with Lot.
Bioactivity ≥ 0.9 pmole/min/µg. Enzyme activity was measured in a buffer containing 100 mM Sodium acetate (pH 5.5), 2 mM EDTA, 8 mM DTT, 0.05% Brij, and 20 µM substrate (Z-FRAMC), with 3.75-30 ng/µl Cathepsin F. Reaction was carried out at 30°C for 40-120 min, and fluorescence signal was measured at Ex360 nm/Em460 nm. Enzyme was preactivated in 50 mM sodium acetate pH 4.5, 5 mM DTT buffer for 90 min at RT prior to reaction.
SwissProt/Accession Q9UBX1
Gene CTSF
Residue 20 to 484
Sequence Belongs to the peptidase C1 family.
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