All lanes : Anti-beta Catenin (phospho S33) antibody ( ab73153 ) at 1 µg/ml Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate ( ab3957 ) Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate with Human beta Catenin (phospho S33) peptide (ab73676) at 1 µg/ml Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human beta Catenin (phospho S33) peptide (ab73676) at 1 µg/ml Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human beta Catenin (phospho S33) peptide (ab73676) at 1 µg/ml Lane 8 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate ( ab3957 ) with Human beta Catenin (phospho S33) peptide (ab73676) at 1 µg/ml Lane 9 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate with Human beta C