Active human Cripto1 protein fragment
| Name | Active human Cripto1 protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab84064 |
| Category | Protein |
| Prices | $340.00 |
| Sizes | 5 µg |
| Applications | SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | ab84064 has been shown to stimulate MAPK phosphorylation in HUVEC cells. 200ng/ml is sufficient to stimulate phosphorylation. |
| SwissProt/Accession | P13385 |
| Gene | TDGF1 |
| Residue | 31 to 169 |
| Sequence | Theoretical sequence: LGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPP MGIQHSKELN RTCCLNGGTCMLGSFCACPPSFYGRNCEHDVRKENCGS VPHDTWLPKKC SLCKCWHGQLRCFPQAFLPGCDGLVMDEHLVASRTPE LPPS |
| Supplier Page | Shop |
Product images
Lane 1- MW markers; Lane 2- ab84064 ; Lane 3- ab84064 treated with PNGase F to remove potential N-linked glycans; Lane 4- ab84064 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie. A drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. O-fucosylation at Thr-88 has been confirmed.