| Name | Active human Cyclophilin 40 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab78815 |
| Category | Protein |
| Prices | $192.00 |
| Sizes | 50 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Purity | > 95 % by SDS-PAGE. ab78815 is purified using conventional chromatography techniques. |
| Bioactivity | Specific activity is > 210 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. Add 10 µl of recombinant Cyclophilin D protein with 1 µg in assay buffer. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) Record the increase in A405 nm for 30 minutes at 25°C. |
| SwissProt/Accession | Q08752 |
| Gene | PPID |
| Sequence | MGSSHHHHHHSSGLVPRGSHMSHPSPQAKPSNPSNPRVFFDVDIGGERVG RIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKGCPFHRIIKKF MIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGS QFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAE CGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIG NTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIG ACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADL KKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA |
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