Name | Recombinant Human FGF-BP Protein, CF |
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Supplier | R&D Systems |
Catalog | 1593-FB |
Category | Protein |
Prices | $259.00 |
Sizes | 25 µg |
Species Reactivities | Human |
Nature | Recombinant |
Source | Mouse myeloma cell line, NS0-derived Lys24-Cys234 & Asn29-Cys234 both with a C-terminal 10-His tag |
Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Bioactivity | Measured by its binding ability in a functional ELISA. 1 μg/mL of rhFGF-BP was mixed with serially diluted rhFGF basic (Catalog # 233-FB). Following incubation, the FGFBP-FGF basic complex was captured on a Gt x hFGFBP-coated plate. Bound FGF basic was measured using biotinylated Gt x hFGF basic. The concentration of rhFGF basic that produces 50% of the optimal binding was found to be approximately 5-20 ng/mL. |
Endotoxin | <1.0 EU per 1 μg of the protein by the LAL method. |
SwissProt/Accession | Q14512 |
Gene | FGFBP1 |
Sequence | Lys24 & Asn29 |
Supplier Page | Shop |
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J. Nature. 2012 Jul 26;487(7408):505-9.
Tassi E, McDonnell K, Gibby KA, Tilan JU, Kim SE, Kodack DP, Schmidt MO, Sharif GM, Wilcox CS, Welch WJ, Gallicano GI, Johnson MD, Riegel AT, Wellstein A. Am J Pathol. 2011 Nov;179(5):2220-32.