| Name | Recombinant Human GASP-1/WFIKKNRP Protein, CF |
|---|---|
| Supplier | R&D Systems |
| Catalog | 2070-GS/CF |
| Category | Protein |
| Prices | $319.00 |
| Sizes | 25 µg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Mouse myeloma cell line, NS0-derived Leu35-His576, with a C-terminal 6-His tag |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Bioactivity | Measured by its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. The ED 50 for this effect is 0.03-0.15 µg/mL, in the presence of 40 ng/mL of rmGDF-8. Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH 2 (Catalog # ES002 ). The IC 50 value is approximately 1.5 nM, as measured in a reaction mixture containing 1.0 nM trypsin,10 mM ES002, 50 mM Tris, 10 mM CaCl 2 , 0.15 M NaCl, pH 7.5. |
| Endotoxin | <0.10 EU per 1 μg of the protein by the LAL method. |
| SwissProt/Accession | Q8TEU8 |
| Gene | WFIKKN2 |
| Sequence | Leu35 |
| Supplier Page | Shop |