| Name | Recombinant Human IBSP/Sialoprotein II Protein, CF |
|---|---|
| Supplier | R&D Systems |
| Catalog | 4014-SP |
| Category | Protein |
| Prices | $329.00 |
| Sizes | 50 µg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Chinese Hamster Ovary cell line, CHO-derived Phe17-Gln317, with a C-terminal 10-His tag |
| Purity | >90%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. When 5 x10 4 cells/well are added to recombinant human IBSP-coated plates (3 µg/mL with 100 µL/well), approximately 60‑80% will adhere after 30 minutes at 37° C. Optimal concentration depends on cell type as well as the application or research objectives. |
| Endotoxin | <1.0 EU per 1 μg of the protein by the LAL method. |
| SwissProt/Accession | AAC95490 |
| Gene | IBSP |
| Sequence | Phe17 |
| Supplier Page | Shop |
Barros NM, Hoac B, Neves RL, Addison WN, Assis DM, Murshed M, Carmona AK, McKee MD. J Bone Miner Res. 2013 Mar;28(3):688-99.
Akter R, Rivas D, Geneau G, Drissi H, Duque G. J Bone Miner Res. 2009 Feb;24(2):283-93.