| Name | Recombinant Mouse Chitinase 3-like 1 Protein, CF |
|---|---|
| Supplier | R&D Systems |
| Catalog | 2649-CH |
| Category | Protein |
| Prices | $329.00 |
| Sizes | 50 µg |
| Species Reactivities | Mouse |
| Nature | Recombinant |
| Source | Mouse myeloma cell line, NS0-derived Tyr22-Ala381, with a C-terminal 6-His tag |
| Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 5 x 10 4 cells per well are added to rmCHI3-L1 coated plate, cell adhesion is enhanced in a dose dependent manner after 30 minutes at 37 °C. The ED 50 for this effect is 0.8-3.2 μg/mL. Optimal concentrations should be determined by each laboratory for each application. |
| Endotoxin | <0.10 EU per 1 μg of the protein by the LAL method. |
| SwissProt/Accession | Q61362 |
| Gene | Chil1 |
| Sequence | Tyr22 |
| Supplier Page | Shop |
Lee CM, He CH, Nour AM, Zhou Y, Ma B, Park JW, Kim KH, Dela Cruz C, Sharma L, Nasr ML, Modis Y, Lee CG, Elias JA. Nat Commun. 2016 Sep 15;7:12752.
Tanaka Y, Matsumoto I, Inoue A, Umeda N, Takai C, Sumida T. Clin Exp Immunol. 2014 Aug;177(2):419-27.