Recombinant Mouse GDF-3 (Catalog # 9009-GD) Induces Smad2 phosphorylation in P19 mouse embryonic carcinoma cells. P19 cells were incubated with 50 and 100 ng/ml of Recombinant Mouse GDF-3 for 60 minutes. The cells were lysed and Western blots were performed with anti-phospho Smad2 and eIF4E which was served as a loading control.
1 μg/lane of Recombinant Mouse GDF-3 (Catalog # 9009-GD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 16 and 17 kDa and NR bands at 16 and 18 kDa.