| Name | Recombinant Human His6-USP12 Protein, CF |
|---|---|
| Supplier | R&D Systems |
| Catalog | E-578 |
| Category | Protein |
| Prices | $329.00 |
| Sizes | 50 µg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Spodoptera frugiperda , Sf 21 (baculovirus)-derived Contains a C-terminal 6-His tag |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
| Bioactivity | Recombinant Human USP12 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP12 concentration of 50-500 nM. Requires a 1:1 stoichiometric amount of UAF1 ( E-566 ) for activity when using Ubiquitin-AMC or Ubiquitin-Rhodamine ( U-550 , U-555) as a substrate. In vitro , the USP12/UAF1 complex will not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20. |
| SwissProt/Accession | O75317 |
| Gene | USP12 |
| Supplier Page | Shop |