SDS PAGE analysis of ab113404: Lane 1: MW Marker, Lane 2: 0.25ug, Lane 3: 0.5ug, Lane 4: 1.0ug, Lane 5: 2.5ug, Lane 6: 5.0ug
All lanes : an anti Human ERp72 antibody Lane 1 : Molecular weight ladder Lane 2 : Active human ERp72 full length protein (ab113404) at 0.025 µg Lane 3 : Active human ERp72 full length protein (ab113404) at 0.05 µg Lane 4 : Active human ERp72 full length protein (ab113404) at 0.1 µg
Activity Assay: Varying amounts of ab113404 were incubated with insulin in the presence of DTT. Activity is measured as an increase in absorbance over time.