All lanes : Anti-CREB (phospho S133) antibody ( ab37714 ) at 1 µg/ml Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human CREB (phospho S133) peptide (ab38313) at 1 µg/ml Lane 4 : NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate with Human CREB (phospho S133) peptide (ab38313) at 1 µg/ml Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human CREB peptide ( ab42655 ) at 1 µg/ml Lane 6 : NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate with Human CREB peptide ( ab42655 ) at 1 µg/ml Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing conditions. Predicted band size : 37 kDa Observed band size : 40 kDa ( why is the actual band size different from the predicted? )