| Name |
Active human FKBPL full length protein
|
| Supplier |
Abcam
|
| Catalog |
ab98117
|
| Category |
Protein
|
| Prices |
$239.00, $883.00
|
| Sizes |
10 µg, 50 µg
|
| Applications |
SDS-PAGE MS FA
|
| Species Reactivities |
Human
|
| Nature |
Recombinant
|
| Source |
E. coli
|
| Tag/Conjugation |
His tag C-Terminus
|
| Purity |
> 90 % by SDS-PAGE. ab98117 is purified by using conventional chromatography techniques.
|
| Bioactivity |
Specific activity is > 210 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. Add 10 µl of recombinant FKBPL protein with 1 µg in assay buffer. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) Record the increase in A405 nm for 30 minutes at 25°C.
|
| SwissProt/Accession |
Q9UIM3
|
| Gene |
FKBPL
|
| Residue |
1 to 349
|
| Sequence |
METPPVNTIG EKDTSQPQQE WEKNLRENLD SVIQIRQQPR DPPTETLELE VSPDPASQIL EHTQGAEKLV AELEGDSHKS HGSTSQMPEA LQASDLWYCP DGSFVKKIVI RGHGLDKPKL GSCCRVLALG FPFGSGPPEG WTELTMGVGP WREETWGELI EKCLESMCQG EEAELQLPGH SGPPVRLTLA SFTQGRDSWE LETSEKEALA REERARGTEL FRAGNPEGAA RCYGRALRLL LTLPPPGPPE RTVLHANLAA CQLLLGQPQL AAQSCDRVLE REPGHLKALY RRGVAQAALG NLEKATADLK KVLAIDPKNR AAQEELGKVV IQGKNQDAGL AQGLRKMFG L EHHHHHH
|
| Supplier Page |
Shop
|
15% SDS-PAGE analysis of ab98117 (3ug)