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Waf1/Cip1/CDKN1A p21 Antibody (F-8): sc-271610

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Datasheets
  • Waf1/Cip1/CDKN1A p21 Antibody (F-8) is a mouse monoclonal IgG2a κ, cited in 38 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 124-164 at the C-terminus of p21 Waf1/Cip1 of human origin
  • Anti-Waf1/Cip1/CDKN1A p21 Antibody (F-8) is recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and porcine
  • Anti-Waf1/Cip1/CDKN1A p21 Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Waf1/Cip1/CDKN1A p21 (F-8): sc-271610.
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Waf1/Cip1/CDKN1A p21 Antibody (F-8) for WB applications. These reagents are now offered in bundles with Waf1/Cip1/CDKN1A p21 Antibody (F-8) (see ordering information below).

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p21 Waf1/Cip1 Antibody (F-8) is a mouse monoclonal IgG2a kappa light chain antibody specific for an epitope mapping between amino acids 124-164 at the C-terminus of p21 Waf1/Cip1 of human origin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p21 Waf1/Cip1, also known as cyclin-dependent kinase inhibitor 1A (CDKN1A) or CAP20 in mice, is a critical regulator of the cell cycle, acting as an inhibitor of cyclin-dependent kinases (Cdks). p21 Waf1/Cip1 binds Cdks to prevent phosphorylation of the retinoblastoma protein (Rb), thereby halting cell cycle progression and promoting cellular responses such as senescence or apoptosis in reaction to DNA damage. p21 Waf1/Cip1 is primarily localized in the nucleus, where p21 Waf1/Cip1 interacts with cell cycle machinery, emphasizing its role in maintaining genomic stability and suppressing tumorigenesis. p21 Waf1/Cip1 expression is directly induced by wildtype p53 in response to cellular stress, linking p21 Waf1/Cip1 to pathways involved in DNA repair and cell survival. anti-p21 Waf1/Cip1 antibody (F-8) is available in both non-conjugated forms and a variety of conjugated formats, including agarose, horseradish peroxidase (HRP), and Alexa Fluor® dyes, enabling diverse experimental applications.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Waf1/Cip1/CDKN1A p21 Antibody (F-8)

sc-271610
200 µg/ml
$316.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8): m-IgG Fc BP-HRP Bundle

sc-529221
200 µg Ab; 10 µg BP
$354.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8): m-IgGκ BP-HRP Bundle

sc-521996
200 µg Ab, 40 µg BP
$354.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8) AC

sc-271610 AC
500 µg/ml, 25% agarose
$416.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8) HRP

sc-271610 HRP
200 µg/ml
$316.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8) FITC

sc-271610 FITC
200 µg/ml
$330.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8) PE

sc-271610 PE
200 µg/ml
$343.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8) Alexa Fluor® 488

sc-271610 AF488
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8) Alexa Fluor® 546

sc-271610 AF546
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8) Alexa Fluor® 594

sc-271610 AF594
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8) Alexa Fluor® 647

sc-271610 AF647
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8) Alexa Fluor® 680

sc-271610 AF680
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 Antibody (F-8) Alexa Fluor® 790

sc-271610 AF790
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 (F-8) Neutralizing Peptide

sc-271610 P
100 µg/0.5 ml
$68.00

What is the best secondary to use with your mouse monoclonal antibodies for WB?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-17
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Rated 5 out of 5 by from Worked excellent on our western blotsWe used it 1:1000 on our immunoblots. The signals were excellent.
Date published: 2021-07-29
Rated 1 out of 5 by from very bad productit is not working in cell lysate especially human cell lines (hepG2)
Date published: 2020-10-22
Rated 5 out of 5 by from Great antibody!Used at 1:200 for mouse tissue lysates. Antibody gave good clear band! Recommend!
Date published: 2018-03-23
Rated 5 out of 5 by from very strong signalSpecific WB band in HeLa whole cell lysate, 1:300 dilution, Good data!!!
Date published: 2017-04-05
Rated 5 out of 5 by from Strong and Specificp21 (F-8) gives a strong specific band for Western blot (WB) at 21 kDa.
Date published: 2016-12-20
Rated 5 out of 5 by from great1:1000 dilution for detection of p21 in DLBCL lymphoma cells, incubate with the filter overnight at 4C. It's very sensitive and stable with the clean background.
Date published: 2016-12-17
Rated 4 out of 5 by from This has worked well in WB with mouse liverThis has worked well in WB with mouse liver.
Date published: 2015-12-07
Rated 5 out of 5 by from This has worked well in WB with mouse liverThis has worked well in WB with mouse liver
Date published: 2015-10-16
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Waf1/Cip1/CDKN1A p21 Antibody (F-8) is rated 4.5 out of 5 by 13.
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