ATF-5 Antibody (E-10): sc-377168
- ATF-5 Antibody (E-10) is a mouse monoclonal IgG1 κ ATF-5 antibody, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 1-83 mapping at the N-terminus of ATF-5 of human origin
- ATF-5 Antibody (E-10) is recommended for detection of ATF-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ATF-5 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-377168 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ATF-5 (E-10): sc-377168.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for ATF-5 Antibody (E-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with ATF-5 Antibody (E-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
ATF-5 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATF-5 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ATF-5 (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATF-5 plays a crucial role in cellular stress responses and is involved in the regulation of gene expression during conditions such as hypoxia and nutrient deprivation. ATF-5′s ability to modulate transcription in response to stress is vital for cell survival and adaptation, making ATF-5 an important target for research in cancer and neurodegenerative diseases. ATF-5 localizes in both the cytoplasm and nucleus, where ATF-5 functions as a dimer to bind DNA, and interacts with other proteins such as CCND3 and PTP4A1, which are involved in cell cycle regulation and signal transduction pathways. The ATF/CREB transcription factor family, to which ATF-5 belongs, is characterized by a conserved C-terminal leucine zipper that facilitates dimerization and DNA binding, while the N-terminal domains exhibit significant variability, allowing for diverse regulatory functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ATF-5 Antibody (E-10) References:
- Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis. | Pati, D., et al. 1999. Mol Cell Biol. 19: 5001-13. PMID: 10373550
- Mouse Atf5: molecular cloning of two novel mRNAs, genomic organization, and odorant sensory neuron localization. | Hansen, MB., et al. 2002. Genomics. 80: 344-50. PMID: 12213205
- DISC1 (Disrupted-In-Schizophrenia 1) is a centrosome-associated protein that interacts with MAP1A, MIPT3, ATF4/5 and NUDEL: regulation and loss of interaction with mutation. | Morris, JA., et al. 2003. Hum Mol Genet. 12: 1591-608. PMID: 12812986
- Distinctive gene expression of human lung adenocarcinomas carrying LKB1 mutations. | Fernandez, P., et al. 2004. Oncogene. 23: 5084-91. PMID: 15077168
- Downregulation of activating transcription factor 5 is required for differentiation of neural progenitor cells into astrocytes. | Angelastro, JM., et al. 2005. J Neurosci. 25: 3889-99. PMID: 15829641
- Regulated expression of ATF5 is required for the progression of neural progenitor cells to neurons. | Angelastro, JM., et al. 2003. J Neurosci. 23: 4590-600. PMID: 12805299
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATF-5 Antibody (E-10) | sc-377168 | 200 µg/ml | $316.00 | |||
ATF-5 Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-523219 | 200 µg Ab, 40 µg BP | $354.00 | |||
ATF-5 Antibody (E-10) X | sc-377168 X | 200 µg/0.1 ml | $316.00 | |||
ATF-5 Antibody (E-10) AC | sc-377168 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ATF-5 Antibody (E-10) HRP | sc-377168 HRP | 200 µg/ml | $316.00 | |||
ATF-5 Antibody (E-10) FITC | sc-377168 FITC | 200 µg/ml | $330.00 | |||
ATF-5 Antibody (E-10) PE | sc-377168 PE | 200 µg/ml | $343.00 | |||
ATF-5 Antibody (E-10) Alexa Fluor® 488 | sc-377168 AF488 | 200 µg/ml | $357.00 | |||
ATF-5 Antibody (E-10) Alexa Fluor® 546 | sc-377168 AF546 | 200 µg/ml | $357.00 | |||
ATF-5 Antibody (E-10) Alexa Fluor® 594 | sc-377168 AF594 | 200 µg/ml | $357.00 | |||
ATF-5 Antibody (E-10) Alexa Fluor® 647 | sc-377168 AF647 | 200 µg/ml | $357.00 | |||
ATF-5 Antibody (E-10) Alexa Fluor® 680 | sc-377168 AF680 | 200 µg/ml | $357.00 | |||
ATF-5 Antibody (E-10) Alexa Fluor® 790 | sc-377168 AF790 | 200 µg/ml | $357.00 |